The small amount of equipment and materials and the simplicity of the method are the most important advantages of this test. In this study methylene blue reduction test and standard plate count plate count Subject Category: Properties
count, and decreasing of spoilage and increasing shelf life of food products these methods have been received more attentions [8,10]. It is confirmed that microwave heating has some Enormous advantages in comparison with current method in reduction of process time and improving foods quality [25]. Because of the lack of essential
Jun 04, 2018 · Spread Plate: Less area to grow. Purpose. Pour Plate: To count the number of colony-forming bacteria in a sample. Spread Plate: To isolate specific clonal colonies. Advantages. Pour Plate: Allows the identification of bacteria as aerobes, anaerobes or facultative aerobes; allows the growth of microaerophiles. Spread Plate: Even distribution of ...
This section provides an overview of the different cell counting methods and automated cell counters available, and mentions their advantages and disadvantages. Related Topics: Transfection, Real-Time PCR and Multiplex Immunoassays.
Explain the standard plate method. Original sample is diluted with bottles or tubes. A set volume (aliquot) of the dilutions is then put onto plates, then the number of colonies on the plates is counted after incubation of 24 hrs or more
coliforms in natural environments (plate counts) was sub-sequently investigated for freshwaters. Finally, experiments were performed to test the validity and the main advantages of the use of rapid enzymatic assays as a surrogate measure of coliform abundance in aquatic systems. MATERIALS AND METHODS Sampling
Oct 24, 2014 · The viable plate count (VPC) is considered as the reference method for bacterial enumeration in periodontal microbiology but shows some important limitations for anaerobic bacteria. As anaerobes such as Porphyromonas gingivalis are difficult to culture, VPC becomes time‐consuming and less sensitive.
The advantages of the membrane filter procedure over the standard plate count method have been described by many investigators.6-8 The volume of inoculum is limited with both pour and spread plate techniques while the membrane filter method enables the use of large samples, which is desirable for water with low counts. Principles of the Procedure
The Aerobic Plate Count (APC) is used as an indicator of bacterial populations on a sample. It is also called the aerobic colony count, standard plate count, Mesophilic count or Total Plate Count. The test is based on an assumption that each cell will form a visible colony when mixed with agar containing the appropriate nutrients.
Figure 2 illustrates the pour plating method in which a separate final dilution tube is prepared for each plate and then the entire contents of the tube are poured onto the agar surface. This method has the advantage of not requiring the use of a flame.
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  • Ingredient supplier Sabinsa used a method called 'flow cytometry' to count the number of surviving probiotics in a product and argued in a published study that it’s a viable method to do so.
  • Wires are the basic element to deliver constant electricity to different places. The metals have good conductivity so that they are used widely for making wires and copper is the first choice of all the electrical applications. However, the copper has great amount of oxygen in it, which leads to rusting very soon. This reduces the longevity and durability of the application. To prevent this ...
  • Conclusion: Despite the fact that the new single-copy tuf gene-based qPCR assay developed here is a specific, rapid, and easy method for quantification of both cultivable and dormant BB-12 cells, it does not distinguish dead and viable cells. Moreover, selective plate count method doesn’t quantify dormant bacterial populations.

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5. The plate count method does have two advantages over other methods, however. Only viable organisms are counted, which are the ones usually considered important. Also, samples with small numbers can be counted, which would have insufficient numbers for other methods. Direct Count In this method of counting organisms, a suspension of bacteria ...

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@article{osti_5324727, title = {Ultraviolet disinfection of potable water}, author = {Wolfe, R L}, abstractNote = {Because of upcoming surface and groundwater regulations regarding the control of microbiological and chemical contaminants, there is a need to evaluate the feasibility and effectiveness of ultraviolet (UV) radiation for primary disinfection of potable water supplies.

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Some disadvantages of this method are: it generally takes a long time to incubate bacteria and osmotic shocks can occur. ... Because plate count agar is still agar. therefore, it still has all the ...

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Plate count, or efficiency as it is also known, increases with column length only if peak width increases at a slower rate than compound retention increases, which is not always the case. In flash chromatography theoretical plates do matter, of course, but only to the extent that you have sufficient selectivity for the separation and use the ...


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TOTAL PLATE COUNT. Many culture methods make use of a solidified medium within a petri plate. The three most important procedures involving in plate counts are: 1) Streak dilution plate. 2) Spread plate. 3) Pour plate. a) Streak dilution plate. Streaking a plate for single colonies is one of the most important basic skills in microbiology.

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By multiplying this count by the total dilution of the solution, it is possible to find the total number of CFUs in the original sample. One major disadvantage of the viable plate count is the assumption that each colony arises from one cell. In species where cells grow together in clusters, a gross underestimation of the true population results.

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Settle Plate Samples. Select suitable agar media for sampling; Place the plates at table-top level and remove the lids; Leave the plates open for 0.5-4 hours; Cover the plates and secure the lids with clear tape; Label the plates with appropriate information

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Plate counting method Step One: Diluting the sample. Depending on the source of the sample used there might be thousands, millions or even billions of microorganisms per millilitre of sample. This is too many for us to count so we dilute the sample. 1ml of sample is added to 9ml of a suitable diluent (e.g. sterile buffer) (Figure 1a).

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The advantages of the membrane filter procedure over the standard plate count method have been described by many investigators. 6-8 The volume of inoculum is limited with both pour and spread plate techniques while the membrane filter method enables the use of large samples, which is desirable for water with low counts.

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Aug 17, 2006 · Limitations of pour plate - because anything and everything will grow in a pour plate, we may have such a crowded plate that we cannot count anything at all. It is for this reason that we use diluted sample across several plates. Testing in duplicate can be handy also. Also try incubating plates at different temperatures.

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May 23, 2014 · A disadvantage of the plate-counting method is that a microorganism colony could arise from one cell or from many cells. The other standard methodology, known as optical density determination, is based on the turbidity of a suspension and registers all bacteria, dead and alive.

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plate-count method for P.fluorescens and indirect ELISA for A. brasilense. The samples were then filtered through Whatman no. 1 filter-paper for the dry -mass determination (60 °C for 16 h). Bacterial characterization and the effects on plants after inoculation with recovered A. brasilense Cd from dry inoculants

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Microgirl, Can you explain me why a 10µl spot of a dilution -1 is a -3 dilution? In my opinion: If you have a bottle(100µl or whatever) with -1 diluted sample and you plate out 10µl of that bottle on a plate and lets say you plated out 1 10µL sample (to make it easy) and you notice 4 colonies then you have 4 colonies per 10µl wich is -1 diluted thus 40 colonies for the undeluted sample.

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Feb 01, 2012 · Among different methods of antisepticising of food, because of protection nutritional value of product, improve the quality of food and decrease of its cost that it is important for customer the non thermal methods which results in significant reduction in microbial count, and decreasing of spoilage and increasing shelf life of food products ...

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Standard Plate Count (SPC) By far the most widely used method for determining the number of viable colony forming units (CFU) in a food. Use a spread or pour plate (psychrotrophs may not survive as well in pour plates) that includes homogenized food sample. Incubation is aerobic at 35 o C for 48± 2 h.

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May 16, 2017 · Advantages: This method is simple, inexpensive, and a good indicator of membrane integrity , and dead cells are colored blue within seconds of exposure to the dye . Disadvantages: Cell counting is generally done using a hemacytometer . Therefore, counting errors (~10%) could be occurred.

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Sep 17, 2013 · The tetrazolium method is especially convenient for researchers who need to perform multiple assays using a plate-based spectrophotometer. However, notes Pam Guthmiller, strategic marketing manager at Promega , which offers such kits, users should be aware that the salts themselves can be cytotoxic when used for extended periods, which can lead ...

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The Disadvantages of Pool Ionization. A swimming pool ionizer cleans and sanitizes pool water by releasing positively charged copper and silver ions into the water to destroy algae and bacteria.

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The 3M Petrifilm E. coli/Coliform Count Plate is a sample-ready-culture-medium system which contains Violet Red Bile (VRB) nutrients, a cold-water-soluble gelling agent, an indicator of glucuronidase activity (BCIG), and a tetrazolium indicator that facilitates colony enumeration in food and beverage samples.

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Two methods are also included in this group, the drop plate method and the paper disc method. These two methods are, in a sense, as similar in comparison with each other as the two methods described previously. In the drop plate method, a drop of the substance to be tested is placed directly on a plate and diffuses into the agar. Azone of ...

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disadvantages of standard plate count this method can only count bacteria which are able to develop under the growing conditions provided thermophiles or dead colonies cannot be counted using this procedure Rules for standard plate counting

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HPC heterotrophic plate count in water test results: aerobic and facultative aerobic bacteria are both detected by the HPC count. The HPC count is not part of most basic water potability tests. You may order this test, for example, as part of diagnosing a known or suspected problem, or to help check for growth of microbial contaminants in ...

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Oct 10, 2015 · The plate count procedure consists of two separate but interrelated manipulations of the culture __ serial dilution and placement of microorganisms in/on a solid medium. I) SERIAL DILUTIONS Microbial numbers in environmental samples may be extraordinarily high.

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Count the colonies on each plate after 3, 4 and 5 days incubation. It is necessary to carry out a microscopic examination in order to distinguish, according to their morphology, the colonies of yeast and moulds from

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The treated bacterial cells, with different chitosan concentrations, were subjected to plate count agar and TTC/formazan assay, as quantitative evaluating methods, and the correlation between methods’ results was calculated (Table 1). High correlation averages were calculated between the two methods results, regarding each individual treatment.

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The treated bacterial cells, with different chitosan concentrations, were subjected to plate count agar and TTC/formazan assay, as quantitative evaluating methods, and the correlation between methods’ results was calculated (Table 1). High correlation averages were calculated between the two methods results, regarding each individual treatment.

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Only count plates with 30-200 colonies per plate. To grow cultures of specific individual bacteria, select discrete colonies from any of the plates, choosing colonies that are well separated from neighboring colonies. Sterilize a spreading loop, then open the plate and touch the loop to an empty spot to cool.

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Settle Plate Samples. Select suitable agar media for sampling; Place the plates at table-top level and remove the lids; Leave the plates open for 0.5-4 hours; Cover the plates and secure the lids with clear tape; Label the plates with appropriate information

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1. Total plate count, spread plate 2. Total plate count, spiral plate 3. Vancomycin resistant bacteria, spread plate 4. Vancomycin resistant bacteria, 4-jspiral plate 5. Total Lactic acid bacteria, MPN 6. Cheese flavor scores 7. Cheese body and texture scores 33 39 40 42 43 44

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The plate count method is commonly used in research experiments, since both quantity and species (after some confirmation tests) can be determined. For this analysis of clostridium, the Reinforced Clostridia Medium (RCM-medium) is often used (Jonsson, 1990). However, this plate count method is not usedfor example, by , dairy industry because it is

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The DAPI method would be higher because the DAPI method would count viable cells and dead cells. Also, DAPI staining is not subject to the nutrient and envirionmental requirements of the viable plate counting technique, so all types of organisms could be counted. The DAPI staining method stains the DNA. 8.

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In this study, we explored the advantages and constraints of this assay over the conventional aerobic plate count (APC) method and further developed methods for detection in real environmental and food matrices. The SERS sandwich assay was able to detect environmental bacteria in pond water and on spinach leaves at higher levels than the APC ...

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plate method compared with the spread plate technique (Table 1). It was sug-gested that heterotrophic bacteria in the aquatic environment are already nutri-tionally stressed, which compounds the "transient warming stress" effects of the heated agar medium. As a result, Klein and Wu stated that "the standard plate count procedure should not be considered

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Standard Plate Count (SPC) By far the most widely used method for determining the number of viable colony forming units (CFU) in a food. Use a spread or pour plate (psychrotrophs may not survive as well in pour plates) that includes homogenized food sample. Incubation is aerobic at 35 o C for 48± 2 h.
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Disadvantages of Pour plate method Preparation for the pour plate method is time consuming compared with streak plate/and or spread plate technique. Loss of viability of heat-sensitive organisms coming into contact with hot agar. The reduced growth rate of obligate aerobes in the depth of the agar.Sep 25, 2020 · Advantages: - 1. Direct counting methods are rapid and simple. 2. The morphology of cells can also be observed when they are counted under the microscope. Disadvantages: - 1. The major disadvantage of this method is that it gives the total cell count which includes both viable and non-viable cells. 2.


The plate count method relies on bacteria growing a colony on a nutrient medium so that the colony becomes visible to the naked eye and the number of colonies on a plate can be counted. To be effective, the dilution of the original sample must be arranged so that on average between 30 and 300 colonies of the target bacterium are grown.